Confocal Microscopy

Drosophila larva. Image by Rogério Gomes; sample courtesy of Debbie Obadofin, SeYeon Chung’s lab.

Chung's Lab

Leica TCS SP8 Confocal Microscope with WLL

The Leica SP8 Confocal White Light Laser system operates from a DMI-8 CS Bino inverted microscope with AFC closed-loop focusing, scanning, and Super Z-Galvo stages, turret cooling, spill protection, and CUDA workstation motorized DIC accessories.

Leica SP8 Confocal

  • UV / 405 nm
  • White Light Laser system (470 to 670 nm + Ar) tunable in steps of 1 nm
  • Argon – Blue (458, 476, 488, 496, and 514nm)

5 channels of Confocal Detection

  • 3 Super Sensitive Spectral Hybrid detectors
  • 2 cooled PMT detectors

  • Tandem Scanner (Variable and Resonant) for imaging at speeds from 1 to 16000Hz
  • Scanning XY-stage for montage imaging
  • Automated DIC for all installed objectives (10x/0.30 dry; 20x/0.75 and 63x/1.20 water immersion; 40x/1.30 and 63x/1.40 oil immersion)
  • Adaptive Focus Control
  • LightGate Imaging via pulsed WLL and HyD detectors helps remove reflected light as well as autofluorescence
  • HyVolution 2 integrated deconvolution during image acquisition for improved contrast and resolution
  • Z-stack for 3-D rendering
  • Brightfield:
    • Transmission
    • Reflection
    • Fluorescence
  • Multi-color simultaneous acquisition
  • Serial acquisition
  • Time-lapse
  • FRET
  • FRAP

Quick Guide

Book now

 

Olympus SpinSR10 Spinning Disk Confocal

Built on an IX83 base, the SpinSR10 is a fantastic spinning disk confocal and excels at rapid image capture. This system utilizes super-resolution components as well as the optical sectioning capability of confocal fluorescence microscopes. The system simultaneously achieves high reliability, fast image acquisition, and low phototoxicity, which cannot be done using conventional methods. Based on optical theory, Olympus Super Resolution (OSR) processing algorithms can obtain a spatial resolution of 120 nm, providing highly reliable results below the diffraction limit. Fast image acquisition is achieved using the spinning disk confocal microscope technique. Using the available super-resolution SoRa disk technology, phototoxicity can be reduced to 30% of conventional techniques. Equipped with an incubation system with temperature and CO2 control for long-term in vivo experiments.

Spinning Disk Confocal

Two Hamamatsu ORCA-Fusion (16-bit grayscale)

Description Power (mW) Wavelength (nm) 
LD405 50 405
LD488 100 488
LD516 100 561
LD640 100 640

  • 50µm
  • SoRa

  • D405/488/561/640
  • D445/514/640

  • B447/60; B525/50; B617/73; B685/40; B535/30
  • B447/60; B525/50

Magnification Objective Type NA Refraction Index
4 UPLFLN PH 0.13

AIR (1.000)
10 UPLXAPOUPLXAPO 0.40

AIR (1.000)
20 UPLXAPO 0.80

AIR (1.000)
40 UPLXAPO 1.40

OIL (1.518)
100 UPLAPO OHR 1.50

OIL (1.518)

Quick Guide

Book Now